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alexa fluor 488 affinipure donkey anti mouse igg  (Jackson Immuno)


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    Structured Review

    Jackson Immuno alexa fluor 488 affinipure donkey anti mouse igg
    Alexa Fluor 488 Affinipure Donkey Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg+antibody/AMCA+AffiniPure+Donkey+Anti-Mouse+IgG/pmc12992974-282-71-79
    Average 93 stars, based on 51 article reviews
    alexa fluor 488 affinipure donkey anti mouse igg - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: Membrane-associated periodic skeleton regulates major forms of endocytosis in neurons through a signaling-driven positive feedback loop
    Article Snippet: The following primary antibodies were used in this study: guinea pig anti-tau antibody 1:500 dilution for IF (Synaptic Systems, 314004), mouse anti-tau antibody 1:500 dilution for IF (BD Biosciences, 556319), guinea pig anti-MAP2 antibody 1:500 dilution for IF (Synaptic Systems, 188004), rabbit anti-MAP2 antibody 1:500 dilution for IF (Synaptic Systems, 188002), chicken anti-neurofascin antibody (R&D system, AF3235), rabbit anti-CHC antibody 1:500 dilution for IF (Abcam, ab21679), rabbit anti-Cav1 antibody 1:400 dilution for IF (Cell Signaling Technology, 3238S), mouse anti-Flot1 antibody 1:100 dilution for IF (BD Biosciences, 610820), mouse anti-endophilinA2 antibody 1:100 dilution for IF (Santa Cruz Biotechnology, sc-365704), mouse anti–αII-spectrin (EnCor Biotechnology, MCA-3D7), mouse anti-βII spectrin antibody 1:200 dilution for IF (Santa Cruz Biotechnology, sc-515592), mouse anti-βII spectrin antibody 1:200 dilution for IF (BD Biosciences, 612563), rabbit anti-adducin antibody 1:500 dilution for IF (Abcam, ab51130), chicken anti-GFP antibody 1:500 dilution for IF (Thermo Fisher Scientific, A10262), rabbit anti-GFP antibody 1:500 dilution for IF (Thermo Fisher Scientific, A11122), goat anti-βIII spectrin antibody 1:100 dilution for IF (Santa Cruz Biotechnology, sc-9660), mouse anti-βIII spectrin antibody 1:100 dilution for IF (Santa Cruz Biotechnology, sc-515737), mouse anti-HA antibody 1:200 dilution for HA-mGluR5a internalization and IF (Thermo Fisher Scientific, 26183), rat anti-NCAM1 (CD56) antibody 1:40 dilution for NCAM1 internalization and IF (Cedarlane, CL10008AP), rat anti-TfR (CD71) antibody 1:500 dilution for IF (Bio-Rad, MCA1033GA), goat anti–LDL receptor (LDLR) antibody 1:100 dilution for IF (Thermo Fisher Scientific, PA5-46987), rabbit anti–phospho-ERK antibody 1:300 dilution (Cell Signaling Technology, 4370S), mouse anti-Aβ42 antibody 1:200 dilution for IF (BioLegend, 805501), and rabbit anti–cleaved caspase-3 (Asp175) antibody 1:400 dilution for IF (Cell Signaling Technology, 9661). .. The following secondary antibodies were used in this study: Alexa Fluor 647–conjugated donkey anti–guinea pig immunoglobulin G (IgG) antibody 1:800 for IF (Jackson ImmunoResearch, 706-605-148), Alexa Fluor 647–conjugated donkey anti–rabbit IgG antibody 1:800 for IF (Jackson ImmunoResearch, 711-605-152), Alexa Fluor 647–conjugated donkey anti–mouse IgG antibody 1:800 for IF (Jackson ImmunoResearch, 715-605-151), Alexa Fluor 647–conjugated donkey anti–rat IgG antibody 1:800 for IF (Jackson ImmunoResearch, 712-165-153), Alexa Fluor 647–conjugated donkey anti–goat IgG antibody 1:800 for IF (Jackson ImmunoResearch, 705-605-147), Cy3-conjugated donkey anti–rabbit IgG antibody 1:800 for IF (Jackson ImmunoResearch, 711-165-152), Cy3-conjugated donkey anti–mouse IgG antibody 1:800 for IF (Jackson ImmunoResearch, 715-165-151), Cy3-conjugated donkey anti–guinea pig IgG antibody 1:800 for IF (Jackson ImmunoResearch, 706-165-148), Cy3-conjugated donkey anti–rat IgG antibody 1:800 for IF (Jackson ImmunoResearch, 712-165-153), Alexa Fluor 488–conjugated donkey anti–mouse IgG antibody 1:800 for IF (Jackson ImmunoResearch, 715-545-150), Alexa Fluor 488–conjugated donkey anti–guinea pig IgG antibody 1:800 for IF (Jackson ImmunoResearch, 706-545-148), Alexa Fluor 488–conjugated donkey anti–rabbit IgG antibody 1:800 for IF (Jackson ImmunoResearch, 711-545-152), donkey anti–mouse IgG antibody (Jackson ImmunoResearch, 715-005-151), donkey anti–rat IgG antibody (Jackson ImmunoResearch, 712-005-153), donkey anti–rabbit IgG antibody (Jackson ImmunoResearch, 711-005-152), and donkey anti–chicken IgG antibody (Jackson ImmunoResearch, 703-005-155) CF583R-conjugated secondary antibodies were made by conjugating the unlabeled secondary antibodies with CF583R succinimidyl ester (Biotium, 96084) to achieve a labeling efficiency of ~2 dyes per antibody, as previously described ( ). .. Plasmids and gene segments used in this study were purchased from Addgene, GeneCopoeia, and Twist Bioscience and subsequently cloned into the lentiviral expression vector FUGW (Addgene, plasmid #14883), through Gibson assembly reaction (New England Biolabs, E5510S) according to the manufacturer’s instructions.

    Article Title: Membrane-associated periodic skeleton regulates major forms of endocytosis in neurons through a signaling-driven positive feedback loop.
    Article Snippet: Lentiviruses were produced by cotransfecting Lenti- X 293T cells with 6 μg of lentiviral expression vector encoding the gene of interest, 4.5 μg of Δ8.9 vector (60) (gift from D. Baltimore, California Institute of Technology), and 3 μg of VSVG packaging vector (Addgene, plasmid #8454) in a 10- cm petri dish using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, L3000008). .. The following secondary antibodies were used in this study: Alexa Fluor 647–conjugated donkey anti–guinea pig immunoglobulin G (IgG) antibody 1:800 for IF (Jackson ImmunoResearch, 706-605-148), Alexa Fluor 647–conjugated donkey anti–rabbit IgG antibody 1:800 for IF (Jackson ImmunoResearch, 711- 605-152), Alexa Fluor 647– conjugated donkey anti–mouse IgG antibody 1:800 for IF (Jackson ImmunoResearch, 715- 605- 151), Alexa Fluor 647–conjugated donkey anti–rat IgG antibody 1:800 for IF (Jackson ImmunoResearch, 712-165-153), Alexa Fluor 647–conjugated donkey anti–goat IgG antibody 1:800 for IF (Jackson ImmunoResearch, 705- 605- 147), Cy3conjugated donkey anti–rabbit IgG antibody 1:800 for IF (Jackson ImmunoResearch, 711- 165- 152), Cy3- conjugated donkey anti– mouse IgG antibody 1:800 for IF (Jackson ImmunoResearch, 715- 165-151), Cy3- conjugated donkey anti–guinea pig IgG antibody 1:800 for IF (Jackson ImmunoResearch, 706- 165-148), Cy3- conjugated donkey anti–rat IgG antibody 1:800 for IF (Jackson ImmunoResearch, 712- 165-153), Alexa Fluor 488–conjugated donkey anti–mouse IgG antibody 1:800 for IF (Jackson ImmunoResearch, 715- 545-150), Alexa Fluor 488–conjugated donkey anti–guinea pig IgG antibody 1:800 for IF (Jackson ImmunoResearch, 706- 545-148), Alexa Fluor 488– conjugated donkey anti–rabbit IgG antibody 1:800 for IF (Jackson ImmunoResearch, 711- 545- 152), donkey anti–mouse IgG antibody (Jackson ImmunoResearch, 715- 005- 151), donkey anti–rat IgG antibody (Jackson ImmunoResearch, 712- 005- 153), donkey anti–rabbit IgG antibody (Jackson ImmunoResearch, 711- 005- 152), and donkey anti–chicken IgG antibody (Jackson ImmunoResearch, 703- 005- 155) CF583R- conjugated secondary antibodies were made by conjugating the unlabeled secondary antibodies with CF583R succinimidyl ester (Biotium, 96084) to achieve a labeling efficiency of ~2 dyes per antibody, as previously described (58). .. Lentivirus production and transduction of hippocampal neurons Plasmids and gene segments used in this study were purchased from Addgene, GeneCopoeia, and Twist Bioscience and subsequently cloned into the lentiviral expression vector FUGW (Addgene, plasmid #14883), through Gibson assembly reaction (New England Biolabs, E5510S) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Pericyte-mediated regulation of angiogenesis during cutaneous wound healing in adult zebrafish
    Article Snippet: After washing with 3% BSA in PBS, the tissues were blocked with blocking buffer (PBS-T containing 3% normal donkey serum and 1% BSA) for 1 h at room temperature, and incubated with mouse anti-GFP antibody (clone JL-8, #632381, Clontech Laboratories, Inc.) at a dilution of 1:500 in blocking buffer for 2–3 days at 4 °C. .. After washing with PBS-T, the skin tissues were incubated with Alexa fluor 488 conjugated donkey anti mouse IgG antibody (#715-545-150, Jackson ImmunoResearch Laboratories Inc.) at a dilution of 1:500 and 2 μM DAPI (Thermo Fisher Scientific) in blocking buffer overnight at 4°C, and washed with PBS-T. .. The stained tissues were mounted with Fluoroshield Mounting Medium (abcam) on glass slides.

    Blocking Assay:

    Article Title: Pericyte-mediated regulation of angiogenesis during cutaneous wound healing in adult zebrafish
    Article Snippet: After washing with 3% BSA in PBS, the tissues were blocked with blocking buffer (PBS-T containing 3% normal donkey serum and 1% BSA) for 1 h at room temperature, and incubated with mouse anti-GFP antibody (clone JL-8, #632381, Clontech Laboratories, Inc.) at a dilution of 1:500 in blocking buffer for 2–3 days at 4 °C. .. After washing with PBS-T, the skin tissues were incubated with Alexa fluor 488 conjugated donkey anti mouse IgG antibody (#715-545-150, Jackson ImmunoResearch Laboratories Inc.) at a dilution of 1:500 and 2 μM DAPI (Thermo Fisher Scientific) in blocking buffer overnight at 4°C, and washed with PBS-T. .. The stained tissues were mounted with Fluoroshield Mounting Medium (abcam) on glass slides.



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